Lentiviral-based vector and its use in directed evolution of genomic regions, genes and polynucleotides
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United States of America Patent
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Dec 13, 2016
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Oct 18, 2012
app pub date -
Nov 10, 2010
filing date -
Nov 10, 2009
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Abstract
A method of directing evolution of a target polynucleotide of interest for obtaining variants of this target polynucleotide, a method to generate genetic variability by preparing a cell library, and a method to isolate or to screen variants of a polynucleotide or variants of a protein able to impact the phenotype of a cell or to confer a desired phenotype to target cells, and to identify theses polynucleotide variants or protein variants responsible for this phenotype are described.
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