Methods for providing sialylated oligosaccharides

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United States of America Patent

PATENT NO 9539270
APP PUB NO 20140336140A1
SERIAL NO

14363856

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Abstract

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The invention relates to a method for providing analogs of human milk oligosaccharides (HMO), in particular oligosaccharides containing terminal sialic acid. The method comprises the steps of: a) providing a source of non-digestible galactooligosaccharides (GOS) containing at least two terminally bonded β-linked galactose residues; b) providing a sialic acid donor having (α2-3)-sialylated O-glycans; c) contacting said GOS with said sialic acid donor in the presence of an enzyme having trans-sialidase activity in an enzyme reaction mixture; and d) isolating from said enzyme reaction mixture a fraction comprising at least 20 percent by weight of disialylated galactooligosaccharides (di-Sia-GOS) based on the dry matter.

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Patent Owner(s)

Patent OwnerAddress
RIJKSUNIVERSITEIT GRONINGENBROERSTRAAT 5 GRONINGEN 9712 CP

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hage, Johannes Adrianus Arkel, NL 2 0
Kamerling, Johannis Paulus Groningen, NL 4 0
Sallomons, Erik Westerbork, NL 2 0
Sanders, Peter Groningen, NL 17 126
van, Vuure Catherina Anna Helmond, NL 2 0
Wilbrink, Maarten Hotse Groningen, NL 4 0

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