Method for highly sensitive detection of protein-protein interaction

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United States of America Patent

PATENT NO 9540678
APP PUB NO 20140141416A1
SERIAL NO

14169750

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Abstract

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The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.

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Patent Owner(s)

  • THE UNIVERSITY OF TOKYO;PROBEX INC.;TOYO BOSEKI KABUSHIKI KAISHA

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Masuda, Kenji Tokyo, JP 66 588
Misawa, Naomi Tokyo, JP 3 1
Miura, Kenji Tokyo, JP 65 914
Nishii, Shigeaki Tokyo, JP 8 7
Okamoto, Tasuku Tokyo, JP 4 10
Ozawa, Takeaki Tokyo, JP 14 26

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