Nucleic acids for down-regulation of gene expression
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United States of America Patent
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Jan 31, 2017
Grant Date -
Jun 25, 2015
app pub date -
Sep 13, 2013
filing date -
Jul 17, 2012
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Abstract
Recombinant nucleic acid molecules are provided that form hair pin structures and can be used to down-regulate gene expression. For example, a nucleic acid molecule can comprise a flanking and lower stem loop sequence from a mir-16 gene; an antisense target sequence; a mir-30 loop sequence; a complement of the anti-sense target sequence; and a lower stem loop complementary to the mir-16 sequence. Methods for down regulating gene expression in a cell using such recombinant nucleic acid molecules are also provided.
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