Method for detecting hydroxylmethylation modification in nucleic acid and use thereof

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United States of America Patent

PATENT NO 9567633
APP PUB NO 20150031552A1
SERIAL NO

14360594

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Abstract

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A method for detecting hydroxymethylation modification in nucleic acid comprises: glycosylating the nucleic acid, digesting with MspI, ligating the digested fragments to a biotin-labeled linker at both ends thereof, digesting with NlaIII; capturing the digested fragments using streptavidin magnetic beads to produce fragments having the biotin-labeled linker at one end and a CATG 4-base sticky end at the other end, wherein these fragments reveal modification information of their adjacent CCGG sites; ligating the CATG sticky end to a linker containing a recognition site of MmeI or Ecop15I, digesting with corresponding restriction endonuclease to produce short sequence fragments that can reveal modification information of their adjacent CCGG sites; and performing a tag number comparison to obtain information about methylation and hydroxymethylation modification relative levels. A use of the method is also provided.

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Patent Owner(s)

Patent OwnerAddress
BGI TECH SOLUTIONS CO LTD518083 1010 BUILDING 7 HUADA COMPREHENSIVE PARK 21 HONG'AN THIRD STREET YANTIAN STREET YANTIAN DISTRICT SHENZHEN GUANGDONG PROVINCE SHENZHEN CITY GUANGDONG PROVINCE 518083

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Gao, Fei Shenzhen, CN 224 1346
Wang, Junwen Shenzhen, CN 6 25
Yang, Huanming Shenzhen, CN 32 145
Zhang, Xiuqing Shenzhen, CN 21 60

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