Luminescent immunoassays for quantitating analytes having a wide concentration range
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United States of America Patent
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Feb 14, 2017
Grant Date -
Oct 17, 2013
app pub date -
Mar 11, 2013
filing date -
Apr 16, 2012
priority date (Note) -
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Abstract
The present invention relates to a method for quantitating an analyte having a wide range concentration in a single assay without having to dilute the sample and repeating the assay. The key feature of the invention is having two cycles of events including sample binding to probe, binding reactions, and detection. After the first cycle of binding and detecting, the probe is dipped into the same sample vessel to bind additional analyte in the sample vessel at a condition that is more favorable to binding than the condition in the first cycle.
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