Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi

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United States of America Patent

PATENT NO 9574199
SERIAL NO

14118995

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Abstract

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The invention relates to a method for the simultaneous integration of two or more copies of a polynucleotide of interest into the chromosome of a fungal host cell comprising at least two pairs of recognition sequences of a site-specific recombinase, each pair flanking a resident negative selection marker; transformation of the cell with a construct carrying a gene of interest also flanked by the recognition sequences to ensure double-crossover events after transient expression of the recombinase, followed by selection for excision of all negative selection markers from the cell.

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  • NOVOZYMES A/S

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Inventor Name Address # of filed Patents Total Citations
Udagawa, Hiroaki Chiba, JP 33 175

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