Enzyme mixture

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United States of America Patent

PATENT NO 9617588
APP PUB NO 20120082981A1
SERIAL NO

13252664

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Abstract

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Polymorphisms are present throughout an organism's genome, and understanding which alleles are present in a particular organism's genome can be advantageous. When probing the identity of these alleles, one must minimize incorrect readings due to inefficiencies in the system. In hydrolysis probe applications, these inefficiencies may be due to over-activity of an exonuclease functionality that excises nucleotides from probes that are only partially, complementary to a region of a target. The present invention provides a mixture that contains a plurality of polymerases including one that has a 5′→3′ exonuclease functionality and one that lacks or substantially lacks it, each in a sufficient relative amount and concentration to increase efficiencies of the system.

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Patent Owner(s)

Patent OwnerAddress
THERMO FISHER SCIENTIFIC BALTICS UAB02241 VILNIUS

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kurkela, Jaakko Espoo, FI 14 23

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