RNase H-based assays utilizing modified RNA monomers
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United States of America Patent
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May 9, 2017
Grant Date -
Apr 17, 2014
app pub date -
Aug 5, 2013
filing date -
Apr 30, 2008
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Abstract
The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3′ end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.
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