Nucleic acid melting analysis with saturation dyes

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United States of America Patent

PATENT NO 9657347
APP PUB NO 20140370507A1
SERIAL NO

14462904

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Abstract

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Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

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Patent Owner(s)

  • BIOFIRE DEFENSE, LLC;UNIVERSITY OF UTAH;UNIVERSITY OF UTAH RESEARCH FOUNDATION AND BIOFIRE DEFENSE, LLC

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bernard, Philip Seth Salt Lake City, US 3 0
Dujols, Virginie Woodstock, US 9 102
Wittwer, Carl T Salt Lake City, US 58 2387
Zhou, Luming Salt Lake City, US 30 278

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