Ultrafiltration for preparing outer membrane vesicles

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United States of America Patent

PATENT NO 9687789
APP PUB NO 20070087017A1
SERIAL NO

10564474

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Abstract

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In place of a step of centrifugation during preparation of outer membrane vesicles (OMVs) from bacteria, the invention utilizes ultrafiltration. This allows much larger amounts of OMV-containing supernatant to be processed in a much shorter time. Thus the invention provides a process for preparing bacterial OMVs, comprising a step of ultrafiltration. The ultrafiltration step is performed on an aqueous suspension of OMVs after they have been prepared from bacteria and the OMVs remain in suspension after the filtration step. The invention is particularly useful for preparing OMVs from Neisseria meningitidis.

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Patent Owner(s)

Patent OwnerAddress
GLAXOSMITHKLINE BIOLOGICALS SARUE DE L'INSTITUT 89 RIXENSART B-1330

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Marsili, Ilio Siena, IT 2 8
Olivieri, Roberto Siena, IT 19 106
Sabbatini, Fabio Siena, IT 7 27

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