High efficiency multiplexed nucleic acid capture in a structured microenvironment
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United States of America Patent
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Jun 27, 2017
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May 21, 2015
app pub date -
Nov 5, 2014
filing date -
Nov 15, 2013
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Abstract
Provided herein is a method for sample analysis. In some embodiments, the method may involve: a) enzymatically attaching a reactive group to nucleic acid molecules in a sample; b) covalently reacting the reactive group with surface exposed reactive sites on a porous support, thereby covalently tethering the nucleic acid molecules to the porous support; c) performing a primer extension reaction using the tethered nucleic acid molecules as a template to produce primer extension products; and d) eluting the primer extension products from the porous support, while leaving the tethered nucleic acid molecules tethered to the porous support.
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