Multi-step separation process
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United States of America Patent
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Jul 4, 2017
Grant Date -
Dec 3, 2015
app pub date -
Jan 9, 2014
filing date -
Jan 9, 2013
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Abstract
The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture, which comprises: (a) purifying the feed mixture in a first chromatographic separation step using an eluent a mixture of water and a first organic solvent, to obtain an intermediate product; and (b) purifying the intermediate product in a second chromatographic separation step using as eluent a mixture of water and a second organic solvent, to obtain the PUFA product, wherein the second organic solvent is different from the first organic solvent and has a polarity index which differs from the polarity index of the first organic solvent by between 0.1 and 2.0, wherein the PUFA product is other than alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), linoleic acid, an ALA mono- di- or triglyceride, a GLA mono- di- or triglyceride, a linoleic acid mono- di- or triglyceride, an ALA C1-C4 alkyl ester, a GLA C1-C4 alkyl ester or a linoleic acid C1-C4 alkyl ester or a mixture thereof.
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