Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 9738861
APP PUB NO 20160060588A1
SERIAL NO

14771358

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Abstract

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A culture system has a culture bag for suspending and culturing pluripotent stem cells in a culture medium; a waste liquid container for storing used culture medium that is connected to the culture bag; a fresh medium container for storing a fresh culture medium that is connected to the culture bag; three-way stopcocks for switching flow channels from the culture bag to the waste liquid container or the fresh culture medium container, etc.; a trap portion for trapping the pluripotent stem cells in the culture medium in the flow toward the waste liquid container side; and a filter portion for subculturing that is formed in fourth and fifth flow channels in parallel to a first flow channel. The first flow channel connects the trap part to the culture bag, and has a mesh by which a cell mass of the pluripotent stem cells can be divided. A pump is used to pump the liquid in the individual flow channels.

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Patent Owner(s)

Patent OwnerAddress
NIPRO CORPORATION3-26 SENRIOKASHINMACHI SETTSU-SHI OSAKA 5668510
KYOTO UNIVERSITY36-1 YOSHIDA-HONMACHI SAKYO-KU KYOTO-SHI KYOTO 606-8501

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Nakatsuji, Norio Kyoto, JP 37 248
Takeuchi, Masakatsu Osaka, JP 20 165
Tateyama, Daiki Osaka, JP 2 6
Yoshikawa, Yoshihiro Osaka, JP 37 146

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