Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells
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United States of America Patent
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Aug 22, 2017
Grant Date -
Mar 3, 2016
app pub date -
Feb 20, 2014
filing date -
Mar 6, 2013
priority date (Note) -
In Force
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Abstract
A culture system has a culture bag for suspending and culturing pluripotent stem cells in a culture medium; a waste liquid container for storing used culture medium that is connected to the culture bag; a fresh medium container for storing a fresh culture medium that is connected to the culture bag; three-way stopcocks for switching flow channels from the culture bag to the waste liquid container or the fresh culture medium container, etc.; a trap portion for trapping the pluripotent stem cells in the culture medium in the flow toward the waste liquid container side; and a filter portion for subculturing that is formed in fourth and fifth flow channels in parallel to a first flow channel. The first flow channel connects the trap part to the culture bag, and has a mesh by which a cell mass of the pluripotent stem cells can be divided. A pump is used to pump the liquid in the individual flow channels.

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- 15 United States
- 10 France
- 8 Japan
- 7 China
- 5 Korea
- 2 Other
Patent Owner(s)
Patent Owner | Address | |
---|---|---|
NIPRO CORPORATION | 3-26 SENRIOKASHINMACHI SETTSU-SHI OSAKA 5668510 | |
KYOTO UNIVERSITY | 36-1 YOSHIDA-HONMACHI SAKYO-KU KYOTO-SHI KYOTO 606-8501 |
International Classification(s)
Inventor(s)
Inventor Name | Address | # of filed Patents | Total Citations |
---|---|---|---|
Nakatsuji, Norio | Kyoto, JP | 37 | 248 |
Takeuchi, Masakatsu | Osaka, JP | 20 | 165 |
Tateyama, Daiki | Osaka, JP | 2 | 6 |
Yoshikawa, Yoshihiro | Osaka, JP | 37 | 146 |
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