TAL-mediated transfer DNA insertion

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United States of America Patent

PATENT NO 9756871
SERIAL NO

14084406

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Abstract

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The invention relates to methods for stably integrating a desired polynucleotide into a plant genome, comprising transforming plant material with a first vector comprising nucleotide sequences encoding TAL proteins designed to recognize a target sequence; transforming the plant material with a second vector comprising (i) a marker gene that is not operably linked to a promoter (“promoter-free marker cassette”) and which comprises a sequence homologous to the target sequence; and (ii) a desired polynucleotide; and identifying transformed plant material in which the desired polynucleotide is stably integrated.

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Patent Owner(s)

Patent OwnerAddress
J R SIMPLOT COMPANY1099 W FRONT ST BOISE ID 83702

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Duan, Hui Boise, US 11 21
Rommens, Caius M Boise, US 7 11
Weeks, J Troy Boise, US 7 25

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