and isoamylase for saccharification
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United States of America Patent
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Sep 19, 2017
Issued Date -
N/A
app pub date -
Dec 5, 2013
filing date -
Dec 14, 2012
priority date (Note) -
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status (Latency Note)
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Abstract
A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
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International Classification(s)
- C12C:BREWING OF BEER
- C12P:FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESI....
- A23L:FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT....
- C12N:MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF
- A21D:TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH FO....
- D06L:BLEACHING, e.g. OPTICAL BLEACHING, DRY-CLEANING, O....
- C11D:DETERGENT COMPOSITIONS
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