Materials and methods for characterizing and using KASIII for production of bi-functional fatty acids
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United States of America Patent
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Nov 7, 2017
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Jan 22, 2014
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Jan 23, 2013
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Abstract
A mutant Bacillus subtilis, which does not express a functional KASIIIA and/or KASIIIB, and method of making; a mutant Rhodospirillum rubrum, which does not express a functional PhaC1, PhaC2, and/or PhaC3, and method of making; method of characterizing substrate specificity of KASIII; method of making mutant KASIII with altered substrate specificity and/or altered level of activity and nucleic acid, vector, host cell/organism, and mutant KASIII; an in vitro, high-throughput spectrophotometric method of assaying KASIII activity; and materials and methods for using KASIII for production of bi-functional fatty acids and the materials so produced.
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