Methods for retrieval of sequence-verified DNA constructs

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United States of America Patent

PATENT NO 9809904
SERIAL NO

13453947

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Abstract

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In some embodiments, methods of recovering a sequence-verified target nucleic acid are provided. In some embodiments, such methods may include tagging each member of a nucleic acid library with a set of adaptor sequences; sequencing the tagged members of the nucleic acid library; and recovering the sequence-verified target nucleic acid from the tagged and sequenced members of the nucleic acid library using a dial-out selection method. In certain embodiments, the members of the nucleic acid library may be tagged with a second set of adaptor sequences.

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Patent Owner(s)

Patent OwnerAddress
UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION4311 11TH AVENUE NE SUITE 500 SEATTLE WA 98105

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hiatt, Joseph Seattle, US 7 258
Kitzman, Jacob Seattle, US 7 45
Patwardhan, Rupali Seattle, US 7 258
Schwartz, Jerrod Seattle, US 5 17
Shendure, Jay Seattle, US 23 723

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