Probe kit for detecting a single strand target nucleotide sequence

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United States of America Patent

PATENT NO 9834812
SERIAL NO

14655276

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Abstract

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There is disclosed a kit for detecting a single strand target nucleotide sequence comprising:

    at least one first nucleic acid probe from 10 to 14 bases, to the 5′ end of which at least one fluorophore is bound;at least one second nucleic acid probe from 35 to 50 bases, comprising, from the 5′ to the 3′ end: a first segment having a nucleotide sequence complementary to the first nucleic acid probe, at least one quencher, and a second segment having a nucleotide sequence complementary to at least part of the target nucleotide sequence, wherein the following relation is met:|ΔG hybr.target3−probe2|>|ΔG hybr.probe1−probe2|.

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Patent Owner(s)

Patent OwnerAddress
FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIAVIA MOREGO 30 GENOVA 1-16163

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Aliberti, Anna Siano, IT 2 0
Battista, Edmondo Nocera Inferiore, IT 1 0
Causa, Filippo Pompei, IT 1 0
Cusano, Angela Maria Caserta, IT 1 0
Netti, Paolo Naples, IT 4 18

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