Method for detecting mutant DNA

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United States of America Patent

PATENT NO 9834813
SERIAL NO

13825223

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Abstract

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The present invention relates to a method for detecting of a mutant DNA using a probe, comprising:

    (1) contacting a sample containing a single-stranded DNA which has a substituted nucleotide, a deleted nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA (wild-type DNA) corresponding thereto with the probe which hybridizes with both single-stranded DNA, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with a wild-type DNA (wild-type hybrid), wherein at least one of the obtained mutant-type hybrid and wild-type hybrid has the stem structure;(2) separating the obtained mutant-type hybrid or/and wild-type hybrid by electrophoresis on the basis of presence or absence of the stem structure or difference in the stem structure; and(3) detecting the presence or absence of the mutant-type DNA in the sample.

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Patent Owner(s)

Patent OwnerAddress
FUJIFILM WAKO PURE CHEMICAL CORPORATION1-2 DOSHOMACHI 3-CHOME CHUO-KU OSAKA 540-8605

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ishikawa, Tomokazu Hyogo, JP 260 2185
Matsukuma, Shoichi Kanagawa, JP 4 1

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