Electrochemical detection of proteases using AC voltammetry on nanoelectrode arrays

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United States of America Patent

PATENT NO 9850520
SERIAL NO

14320215

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An electrochemical method for measuring the activity of enzymes using nanoelectrode arrays fabricated with vertically aligned carbon nanofibers. Short peptide substrates specific to disease-related enzymes are covalently attached to the exposed nanofiber tips. A redox moiety, such as ferrocene, can be linked at the distal end of the nanofibers. Contact of the arrays with a biological sample containing one or more target enzymes results in cleavage of the peptides and changes the redox signal of the redox moiety indicating the presence of the target enzymes.

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Patent Owner(s)

  • KANSAS STATE UNIVERSITY RESEARCH FOUNDATION

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hua, Duy Manhattan, US 3 53
Li, Jun Manhattan, US 1264 16367
Prior, Allan Manhattan, US 1 3
Swisher, Luxi Manhattan, US 1 3
Syed, Lateef Uddin Portland, US 1 3

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