Method for nucleotide detection

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United States of America Patent

PATENT NO 9856529
SERIAL NO

14729376

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Abstract

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A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

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Patent Owner(s)

Patent OwnerAddress
ILLUMINA INC5200 ILLUMINA WAY SAN DIEGO CA 92122

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hall, Kevin Nr Saffron Walden, GB 34 203
Klausing, Kay San Diego, US 8 31
Moore, John San Diego, US 96 936
Shen, Min-Jui Richard Poway, US 42 566
Smith, Vincent Peter Nr Saffron Walden, GB 16 25

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