RNA-mediated gene assembly from DNA oligonucleotides

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United States of America Patent

PATENT NO 9873900
APP PUB NO 20130225446A1
SERIAL NO

13763009

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Abstract

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The present invention is directed to methods and materials for RNA-mediated gene assembly from oligonucleotide sequences. In some embodiments, the oligonucleotides used for gene assembly are provided in an array format. An RNA polymerase promoter is appended to surface-bound oligonucleotides and a plurality of RNA copies of each oligonucleotide are then produced with an RNA polymerase. These RNA molecules self-assemble into a desired full-length RNA transcript by hybridization and ligation. The resulting RNA transcript may then be converted into double strand DNA useful in a variety of applications including protein expression.

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Patent Owner(s)

Patent OwnerAddress
WISCONSIN ALUMNI RESEARCH FOUNDATION614 WALNUT STREET 13TH FLOOR MADISON WI 53726

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Smith, Lloyd Madison, US 14 206
Wu, Cheng-Hsien Madison, US 195 5583

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