Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
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United States of America Patent
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Feb 20, 2018
Grant Date -
Mar 26, 2015
app pub date -
Mar 13, 2013
filing date -
Mar 13, 2012
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Abstract
The present invention is directed to methods and compositions for adding tails of specific lengths to a substrate polynucleotide. The invention also contemplates methods and compositions for immobilization of tailed substrates to a solid support. The disclosure contemplates that the attenuator molecule is any biomolecule that associates with a tail sequence added to a substrate polynucleotide and controls the addition of a tail sequence to the 3′ end of the substrate polynucleotide. The sequence that is added to the substrate polynucleotide is referred to herein as a tail sequence, or simply a tail, and the process of adding a nucleotide to a substrate polynucleotide is referred to herein as tailing.
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