Compositions and methods for the expression of CRISPR guide RNAs using the H1 promoter

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United States of America Patent

PATENT NO 9907863
SERIAL NO

14951240

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Abstract

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The presently disclosed subject matter provides compositions and methods for the expression of CRISPR guide RNAs using the H1 promoter. In particular, compositions and methods are provided for the use of the H1 promoter to express CRISPR guide RNA (gRNA) with altered specificity of the 5′ nucleotide, as well as use of the H1 promoter sequence as a bidirectional promoter to express Cas9 nuclease and the gRNA simultaneously. Compositions and methods are also provided for the expression and regulation of gRNA expression in vivo through the use of RNA ribozymes and regulatable aptazymes.

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Patent Owner(s)

  • THE JOHNS HOPKINS UNIVERSITY

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Jaskula-Ranga, Vinod Baltimore, US 11 47
Zack, Donald Baltimore, US 12 103

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