Efficient deep sequencing and rapid genomic speciation of RNA viruses (vRNAseq)

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United States of America Patent

PATENT NO 9909176
SERIAL NO

14841981

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Abstract

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A method for limited input whole genome sequencing of RNA viruses includes isolating a viral RNA sample, converting the viral RNA sample to a double-stranded viral cDNA sample, constructing a double-stranded viral cDNA amplicon library from the double-stranded viral cDNA sample, and sequencing the double-stranded viral cDNA amplicon library to obtain a double-stranded viral cDNA sample sequencing read.

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Patent Owner(s)

  • THE JOHNS HOPKINS UNIVERSITY

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Evans, Jared D Ellicott City, US 3 2
Mehoke, Thomas S Washington, US 1 0
Thielen, Peter M Ellicott City, US 2 0
Wolfe, Joshua T Bethesda, US 4 5

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