Small RNA capture, detection and quantification

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United States of America Patent

PATENT NO 9920360
SERIAL NO

14233711

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Abstract

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Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

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Patent Owner(s)

  • LIFE TECHNOLOGIES CORPORATION

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chen, Caifu Palo Alto, US 73 1169
Dong, Shoulian Mountain View, US 44 836
Liu, Chunmei Palo Alto, US 216 950
Wong, Linda San Bruno, US 13 382
Wu, Yalei Foster City, US 8 58

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