Constructing method of high-throughput sequencing library and use thereof

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 9920363
APP PUB NO 20140329697A1
SERIAL NO

14358674

Stats

ATTORNEY / AGENT: (SPONSORED)

Importance

Loading Importance Indicators... loading....

Abstract

See full text

The present invention provides a method for constructing a high-throughput sequencing library, which comprises: fragmenting genomic DNA; end-repairing the DNA fragments; adding a base A to the 3′ end of the end-repaired DNA fragments; connecting the DNA fragments having cohesive end A with a methylated adapter; carrying out hybrid capture on the connection products by using specific probes to obtain object fragments; treating the object fragments with bisulfite, to convert non-methylated cytosines to uracils; PCR amplifying the converted object fragments; and separating and purifying the amplification products, wherein the amplification products constitute the high-throughput sequencing library. The present invention also provides a method and an apparatus for identifying methylation information in specified genome regions of a sample.

Loading the Abstract Image... loading....

First Claim

See full text

Family

Loading Family data... loading....

Patent Owner(s)

Patent OwnerAddress
BGI TECH SOLUTIONS CO LTD518083 1010 BUILDING 7 HUADA COMPREHENSIVE PARK 21 HONG'AN THIRD STREET YANTIAN STREET YANTIAN DISTRICT SHENZHEN GUANGDONG PROVINCE SHENZHEN CITY GUANGDONG PROVINCE 518083

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Gao, Fei Shenzhen, CN 224 1346
Jiang, Hui Shenzhen, CN 143 788
Wang, Junwen Shenzhen, CN 6 25
Wang, Tong Shenzhen, CN 158 516
Wu, Honglong Shenzhen, CN 2 10
Wu, Jinghua Shenzhen, CN 7 10

Cited Art Landscape

Load Citation

Patent Citation Ranking

Forward Cite Landscape

Load Citation

Maintenance Fees

Fee Large entity fee small entity fee micro entity fee due date
7.5 Year Payment $3600.00 $1800.00 $900.00 Sep 20, 2025
11.5 Year Payment $7400.00 $3700.00 $1850.00 Sep 20, 2029
Fee Large entity fee small entity fee micro entity fee
Surcharge - 7.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge - 11.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge after expiration - Late payment is unavoidable $700.00 $350.00 $175.00
Surcharge after expiration - Late payment is unintentional $1,640.00 $820.00 $410.00