Single molecule loading methods and compositions
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United States of America Patent
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Jun 12, 2018
Issued Date -
May 21, 2015
app pub date -
Nov 4, 2014
filing date -
Mar 31, 2008
priority date (Note) -
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Abstract
Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided. For example, methods are presented for providing a surface comprising the plurality of array regions by exposing the surface to a solution comprising polymerase enzymes where each polymerase enzyme is bound to a binding structure having several functional moieties. The functional moieties of the binding structure react with the binding elements on the array regions such that the functional moieties on the binding structure react with other available binding sites in an array region, preventing other polymerase-binding structures from loading, and resulting in a single polymerase molecule bound to each of these regions.
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