Membrane filter agar medium for simultaneous detection of total coliforms and E. coli
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United States of America Patent
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Oct 23, 2001
Issued Date -
N/A
app pub date -
Aug 5, 1999
filing date -
Nov 18, 1991
priority date (Note) -
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Abstract
An improved method for detection of total coliforms and E. coli comprising a broth containing an ingredient that will encourage growth and repair of injured coliforms, buffers to maintain a pH in the range of 6.5-8, at least one agent that suppresses growth of gram positive cocci and spore-forming organisms, at least one active agent that will suppress growth of non-coliform gram negative bacteria, and at least one chromogen or fluorogen has been used effectively and is cost effective. In the preferred embodiment, both a fluorogen and chromogen were used. Preferred methods include use of filter and/or plates containing the growth-promoting ingredients and the indicators.
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