Proteins and methods for producing the proteins
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United States of America Patent
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Apr 17, 2007
Grant Date -
May 2, 2002
app pub date -
Apr 17, 1998
filing date -
Feb 20, 1995
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.
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