Apparatus for determining cell membrane dielectric breakdown
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United States of America Patent
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Jan 11, 1983
Grant Date -
N/A
app pub date -
Jan 13, 1981
filing date -
Jan 13, 1981
priority date (Note) -
Expired
status (Latency Note)
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Abstract
An apparatus which enables cell membrane dielectric breakdown to be determined. This determination is essential for the rapid inexpensive, reliable, in vitro quantification of lymphocyte tumor-cell cytotoxicity, lymphocyte membrane fluidity and the analysis of abnormal or tumor cells. The apparatus incorporates therein a cell dispenser used in conjunction with a DC electric field exposure tube. Electronic circuitry and its associated components process, acquire and store cell voltage signals as the cells are drawn through the electric field exposure tube. The procedure utilized with the apparatus of this invention involves quantifying the breakdown voltage required to electrically collapse the cell membrane. The electrical collapse is termed a dielectric breakdown and is characterized by a reversible drop in the cell's resistivity.
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