Promoters from chlorella virus genes providing for expression of genes in prokaryotic and eukaryotic hosts
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United States of America Patent
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Jun 26, 2001
Grant Date -
N/A
app pub date -
Jul 31, 1996
filing date -
Aug 19, 1992
priority date (Note) -
Expired
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Abstract
The invention is directed to novel promoters or mutants thereof from Chlorella virus DNA methyltransferase genes. A Chlorella virus gene promoter is operably linked to a first and/or second DNA sequence encoding a gene that is different from the Chlorella virus gene to form an expression cassette. An expression cassette can be introduced into prokaryotic and/or eukaryotic cells and can provide for a high level of expression of the gene encoded by the first and/or second DNA sequence. The invention also provides a method for screening other Chlorella virus genes for promoters that can function to express a heterologous gene in prokaryotic and/or eukaryotic hosts.
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