Blotting technique for membrane assay
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United States of America Patent
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Oct 13, 1992
Grant Date -
N/A
app pub date -
Aug 22, 1989
filing date -
Aug 22, 1989
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A technique for passaging liquid through a membrane putatively containing, in its interstices, at least one substance for which detection is desired, comprises positioning donor and acceptor bibulous matrices onto either surface of the membrane and squeezing the resulting sandwich. This technique permits the application of small volumes of reagents or wash to the membranes and the facile recovery of the waste. The subject membranes are obtained, for example, by blotting developed electrophoresis gels or directly by their use as supports for specific binding assays.
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