Certain pyridyl-di-sulfide-alkylenecarbonxylate-ortho-nitro-phenylsulfonic acid esters useful for coupling biological materials
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United States of America Patent
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Jul 24, 1990
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N/A
app pub date -
Sep 10, 1987
filing date -
Mar 10, 1984
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Expired
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Abstract
Coupling of biological materials is disclosed via 4-hydroxy-3-nitrobenzene sulfonic acid sodium salt (HNSA) esters. The novel activated esters are structured so as to react at one end with an amine group of a selected biological or non-biological material, thereby releasing the spectroscopically monitorable HNSA dianion, and at the other end with, typically, a sulfhydryl group of a second material which may or may not be biological. The esters provide for a cross-linking reaction that may be easily controlled and monitored.
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