In situ assay of amplified intracellular mRNA targets

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United States of America Patent

PATENT NO 5506105
SERIAL NO

08216233

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Abstract

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In an in situ hybridization assay, cells are fixed under conditions which preserve essential morphology, followed by transcription-based nucleic acid amplification of intracellular mRNA targets. The amplified targets are visualized by hybridizing labelled probes to the target sequences. The assay thereby provides a convenient method for detecting specific gene expression associated with morphologic characteristics of diagnostic significance.

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Patent Owner(s)

  • DADE BEHRING INC.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Haydock, Paul V Seattle, WA 13 230

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