Enhanced plant cell transformation by addition of host genes involved in T-DNA integration
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United States of America Patent
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Oct 2, 2007
Grant Date -
Jun 9, 2005
app pub date -
Jan 26, 2004
filing date -
Sep 15, 1999
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Adding at least one gene involved in plant host cell T-DNA integration enhances transformation by Agrobacterium. The histone H2A gene encoded by the Arabidopsis RAT5 gene increases transformation frequencies of plants, most likely by causing overexpression of a product needed for T-DNA integration. Agrobacterium tumefaciens genetically transforms plant cells by transferring a portion of the bacterial Ti-plasmid, designated the T-DNA, to the plant, and integrating the T-DNA into the plant genome. However, not all plants are transformable by Agrobacterium and transformation frequencies may be too low to be useful. Little is known about the T-DNA integration process, and no plant genes involved in integration have been identified prior to the present invention.
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