Method for producing by cell-free protein synthesis system using thioredoxin-fused protein expression vector
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United States of America Patent
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Aug 7, 2007
Grant Date -
Nov 17, 2005
app pub date -
Nov 19, 2004
filing date -
May 22, 2002
priority date (Note) -
Expired
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Abstract
We claim a method of expression a thioredoxin-fused membrane protein comprising expressing said thioredoxin-fused membrane protein in a cell-free protein synthesis system in the presence of a non-ionic detergent. It is preferable that the fused membrane protein which is a highly hydrophobic protein, e.g., a G protein-coupled receptor, can be produced in a state being highly soluble and capable of forming a biologically active three-dimensional structure. Also, this invention provides a recombinant polynucleotide encoding said thioredoxin-fused membrane protein which is expressed in the cell-free protein synthesis system.
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