Process for improved protein expression by strain engineering
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United States of America Patent
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Dec 10, 2013
Grant Date -
May 25, 2006
app pub date -
Jul 26, 2005
filing date -
Jul 26, 2004
priority date (Note) -
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Abstract
This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.
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