Transposase-mediated imaging of the accessible genome

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United States of America Patent

PATENT NO 11680253
APP PUB NO 20190071656A1
SERIAL NO

16081381

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Abstract

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Methods for labeling and imaging the accessible genome using a transposase are disclosed. In some embodiments, a bifunctional transposase complex or transposome is used to insert adaptors comprising chemical tags selectively at accessible sites in the genome where active regulatory DNA is located. Various chemical tags can be used for labeling DNA at insertion sites, including, for example, fluorescent dyes for fluorescence imaging, metal particles for electron microscopy or magnetic manipulation of DNA, isotopic labels, or biotin or other ligands, haptens, substrates, or inhibitors that are recognized by streptavidin, antibodies, enzymes, or receptors. Labeling DNA in this manner can be used to provide spatial information regarding the positioning of regulatory DNA in the genome and makes possible the imaging and sorting of cells based on the status of their regulatory DNA.

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Patent Owner(s)

Patent OwnerAddress
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITYOFFICE OF THE GENERAL COUNSEL BUILDING 170 THIRD FLOOR MAIN QUAD P O BOX 20386 STANFORD CA 94305-2038

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Buenrostro, Jason Redwood City, US 6 267
Chang, Howard Y Palo Alto, US 31 698
Chen, Xingqi Palo Alto, US 3 84
Greenleaf, William J Stanford, US 41 660

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