Methods for direct fluorescent antibody virus detection in liquids
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United States of America Patent
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Aug 23, 2011
Grant Date -
Oct 14, 2010
app pub date -
Apr 16, 2009
filing date -
Apr 16, 2007
priority date (Note) -
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Abstract
The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.
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