Method of measuring the luminescence emitted in a luminescent assay
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United States of America Patent
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Mar 1, 2005
Grant Date -
Nov 28, 2002
app pub date -
Nov 13, 2001
filing date -
Jan 27, 1992
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention relates to a method of measuring the luminescence emitted in a luminescent assay, which makes it possible to correct certain perturbations due to the measuring medium. The method comprises employing at least one luminescent tracer compound and a luminescent compound used as an internal reference, which, when exposed to the same excitation wavelength, are capable of emitting at different wavelengths, .lambda..sub.1 and .lambda..sub.2 respectively, either by direct luminescence or by the induction of a luminescent emission, and correcting the measurement of the luminescence emitted by the tracer compound at wavelength .lambda..sub.2 on the basis of the measurement of the luminescence emitted by the reference compound at wavelength .lambda..sub.1. The invention further relates to the use of the method in a homogeneous method of detecting and/or determining an analyte, and to a device for carrying it out.
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