Method for the combined isolation of magnet-beads from a liquid sample and subsequent thermocyclisation for the polymerase chain reaction (PCR) and associated arrangement
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United States of America Patent
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Mar 31, 2009
Grant Date -
Nov 15, 2007
app pub date -
Oct 14, 2005
filing date -
Oct 15, 2004
priority date (Note) -
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Abstract
Embodiments generally relate to a PCR wherein a magnetic field is focused in a localized area and also to the possibility of a cyclic transfer of heat. According to at least one embodiment of the invention, a magnetic field gradient is constructed along the direction of flow and the transfer of heat is carried out in a manner perpendicular to the direction of flow. Suitable things are provided therefore. The magnetic field is, in particular, focused by Mumetal® which is arranged on both sides of the flow channel and the temperature is controlled by way of Peltier elements, cooling bodies, and thermal coupling plates.
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