Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A
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United States of America Patent
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Aug 18, 1998
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Jan 16, 1996
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Jan 11, 1996
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Abstract
Mutants of 2,5-diketo-D-gluconic acid reductase A an enzyme used to produce 2-keto-L-gluconic acid, a precursor of ascorbic acid (vitamin C), are prepared by site-directed mutagenesis. These mutants may exhibit one or more of the following characteristics: improved temperature stability, increased resistance to substrate inhibition, increased turnover of the substrate by the enzyme and increased affinity for the substrate.
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