Bacillus thuringiensis transformation method

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United States of America Patent

PATENT NO 5858745
SERIAL NO

08957488

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Particularly effective B.t. insecticides are obtained by culturing to the sporulation stage novel transformant obtained on transforming B.t. kurstaki H.D. 562 with DNA expressing in the B.t. certain mutant endotoxin genes. Such transformants and other B.t. transformants generally may be obtained in high transformation frequencies by the electrotransformation of B.t. cells while in a hypertonic state followed by maintaining the cells in a hypertonic medium for a time sufficient to obtain intact cells. Transformation of B.t. tenebroinis and B.t. aizawai is also described.

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  • NOVARTIS FINANCE CORPORATION

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Beerman, Noah D Cambridge, MA 1 0
Jellis, Cindy Lou Londonberry, NH 1 0
Piot, Jean-Christophe Menlo Park, CA 2 70

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