Melting curve analysis with exponential background subtraction
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United States of America Patent
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Nov 29, 2011
Grant Date -
Sep 3, 2009
app pub date -
Sep 20, 2006
filing date -
Sep 20, 2005
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Abstract
A system and methods are provided for melting curve genotyping analysis of nucleic acids. Melting curves are generated by plotting fluorescence of a sample as a function of temperature. In one illustrative example, an exponential algorithm is employed to remove the background from generated melting curves and thereby perform comparative analysis to other melting curves. Additional illustrative examples provide for measuring the differences between two or more melting curves and clustering the genotypes of the provided sample nucleic acids.
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