Engineered proteases for affinity purification and processing of fusion proteins
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United States of America Patent
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Nov 2, 2010
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Jun 29, 2004
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Aug 6, 2003
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Abstract
The present invention is directed to the identification of a protease prodomain that is capable of binding a corresponding protease with high affinity. The protease prodomain of the present invention is fused to a second protein to form a protease prodomain fusion protein. The presence of a protease prodomain protein in a fusion protein allows for easy and selective purification of the second protein by incubation with the corresponding protease.
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