Primers for obtaining highly informative DNA markers
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United States of America Patent
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May 9, 2000
Grant Date -
N/A
app pub date -
Jul 17, 1997
filing date -
Jul 17, 1997
priority date (Note) -
Expired
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Abstract
Disclosed is a DNA amplification primer pair for the amplification of Sextolet 900 marker, which comprises R14B/264 primer having the sequence of SEQ ID NO:1 and a primer designed from the nucleic acid sequence of Sextolet 900 marker (SEQ ID NO:2). A method for the DNA fingerprinting identification of genetically related or unrelated individuals, which comprises the steps of performing DNA amplification of genomic DNA samples collected from individuals, using the primer pair R14B/264 and Sext.900E (SEQ ID NO:1 and SEQ ID NO:3, respectively) and separating the amplified DNA segments obtained whereby Sextolet 900 marker, having a heterozigosity of at least 0.97 and comprising more than 64 alleles, is amplified and serves as DNA fingerprinting of the individuals.
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