Methods of detection of a target nucleic acid sequence
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United States of America Patent
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Jun 15, 2010
Grant Date -
Dec 20, 2007
app pub date -
Nov 29, 2006
filing date -
Oct 29, 1999
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Abstract
The present invention is directed to methods of generating a signal indicative of the presence of a target nucleic acid in a sample by forming a cleavage structure comprising duplex and single-stranded nucleic acid by incubating a sample comprising a target nucleic acid with a thermostable nucleic acid polymerase substantially lacking 5′ to 3′ exonuclease activity and cleaving said cleavage structure with a thermostable Fen nuclease lacking 5′ to 3′ synthetic activity to generate a signal.
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