Methods of detecting analytes

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United States of America Patent

PATENT NO 11788122
APP PUB NO 20230100497A1
SERIAL NO

18047092

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Abstract

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Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

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Patent Owner(s)

  • 10X GENOMICS SWEDEN AB

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Frisen, Jonas Stockholm, SE 45 2264
Lundeberg, Joakim Stockholm, SE 35 2327
Stahl, Patrik Stockholm, SE 25 1513

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